Development of species specific RAPD markers using populus cpDNA

• 발행기관: 한국육종학회
• 저자명: Jae Soon Lee(李載順)Eun Woon Noh(盧銀雲) Suk Sung Jang(張錫成) Suk Koo Lee(李錫求) Eui Rae Noh(盧義來) Don Koo Lee(李敦求)
• 간행물 정보: 『한국육종학회지』Vol.26 No.4, 335~339쪽, 전체 5쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 1994.12.30

영문 초록

Chloroplast DNAs(cpDNA) from eight different species of Populus(P. alba, P. glandulosa, P. alba×P. glandulosa, P. davidiana, P. maximowiczii, P. nigra×P. maximowiczii, P. nigra, and P. Koreana×P. nigra), Salix pseudo-lasiogyne and Nicotiana tabacum were compared by PCR amplification. The cpDNAs were amplified at relatively low annealing temperature so that many bands could be observed. Amplification of a spacer between 16S γRNA gene and 23S γRNA gene located in inverted repeats in cpDNA resulted in a major band in size of 2.3 Kb and several minor bands of various sizes. A band specific to Populus was found when the amplification was done at lower annealing temperature. Amplification with primers deduced from γpoC1 and γpoC2 gene produced the major target band as well as several additional (minor) bands that could be used for the differentiation of each Populus species. The minor bands appeared repeatedly even when different thermocycles were employed. With the amplification profile, it was possible to differentiate Populus species.

목차

ABSTRACT

INTRODUCTION

MATERIALS AND METHODS

RESULTS AND DISCUSSION

CONCLUSION

摘要

LITERATURE CITED

키워드

cpDNA Populus RAPD 16S rRNA 23S rRNA PCR Marker