Crystallization and preliminary X-ray diffraction analysis of a redox-sensing repressor Rex from Thermotoga maritima

• 발행기관: 한국구조생물학회
• 저자명: Young Yoon Jang Young Woo Park Hyun Kyu Joo Jae Young Lee
• 간행물 정보: 『Biodesign』Vol 5, No 3, Sep, 103~106쪽, 전체 4쪽
• 주제분류: 자연과학 > 생물학
• 파일형태: PDF
• 발행일자: 2017.09.30

영문 초록

The redox-sensing repressor Rex is a homodimeric transcriptional regulator involved in expression of respiratory genes. Because nicotinamide adenine dinucleotide (NAD) exists oxidized or reduced form by catabolic metabolism, intracellular NAD + /NADH ratio can be a key signal indicating the cellular redox state. The Rex from hyperthermophilic bacterium, Thermotoga maritima (TmRex), was cloned and overexpressed in Escherichia coli. The TmRex is composed of 208 amino-acid residues with a molecular mass of 22,954 Da. The TmRex crystals were obtained by the sitting-drop vapourdiffusion method and diffracted to 1.95 Å resolution. The crystals belonged to the monoclinic space group P2 1 , with unitcell parameters a = 53.54 Å, b = 88.34 Å, c = 87.84 Å, and β = 96.74°. Two dimeric molecules of TmRex were present in an asymmetric unit, giving a solvent contents of 45.17%.

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INTRODUCTION

RESULT AND DISCUSSION

METHODS