海洋細菌을 利用한 EPA(eicosapentaenoic acid) 生成에 관한 硏究

• 영문명: Studies on the EPA(eicosapentaenioc acid) production by marine bacteria
• 발행기관: 한국응용과학기술학회 (구.한국유화학회)
• 저자명: 조용계(Joh Yong Goe)(趙鏞桂) 김성진(Kim Seong Jin)(金成眞) 김지수(Kim Ji Soo)(金智修) 이민경(Lee Min Kyeng)(李民京)
• 간행물 정보: 『한국응용과학기술학회지』한국유화학회지 제12권 제2호, 121~130쪽, 전체 10쪽
• 주제분류: 공학 > 화학공학
• 파일형태: PDF
• 발행일자: 1995.11.30

영문 초록

This project has been worked out for isolation of EPA-producing bacteria from marine source of sea water, sea sediment and intestinal contents eviscerated from some red-muscle fish such as mackerel, horsemackerel and spike fish. The samples were precultured on the media of PPES-II glucose broth and then pure-cultured on Nutrient agar and P-Y-M glucose. Lipids extracted from those bacterial mass collected by centrifugation were analysed in terms of lipid class and fatty acid composition. The results are resumed as follows : 1. 112 strains from sea water and 76 strains from sea sediment were tested for their EPA producing capability, but both strains of(SA-67 and SA-91) from the former and four strains(SS-35, 37, 51 and 71) from the latter have been proved to produce EPA above the level of 2% of total fatty acids. The strains such as GS-11, 29, 31, HM-9, 29, B-l8, 33, 107, YL-129, 156, 203, 77, 104 and 256 which were isolated from fish intestinal contents, have also produced EPA at higher level than 2% of total fatty acids. 2. Contents of total lipids extracted from the cultures of these strains grown at 25℃, range from 2.8% to 6.9% (on dry weight %), and they are mainly composed of polar lipids(40.9~52.9%) such as phosphatidyl glycerol(+cardiolipin)(?) and phosphatidyl ethanolamine (33.8~40.0%), with smaller amount of free fatty acid (l1.2~20.2%). 3. EPA was isolated from a mixture of fatty acid methyl esters obtained from the lipid of each strain by HPLC in silver-ion mode and was identified by GC-Mass spectrometry. 4. The strains of SW-91, GS-11, GS-29, HM-9, B-18 and YL-203 grown at 2℃ have a level of 5% EPA in their total fatty acids, and the GS-11 and HM-9 strains show a tendency of increase in the EPA level with an increase of growth temperature.

목차

ABSTRACTS

I. 序論

II. 材料 및 實驗方法

III. 結果 와 考察

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