사철쑥과 그늘쑥의 다당류 연구

• 영문명: Characterization of Polysaccharides from Artemisia capillaris and Artemisia sylvatica
• 발행기관: 대한약학회
• 저자명: 황은주(Eun Ju Hwang) 권학철(Hak Cheol Kwon) 정칠만(Chil Man Jung) 문형인(Hyung In Moon) 김선여(Sun Yeou Kim) 지옥표(Ok Pyo Zee) 이강노(Kang Ro Lee)
• 간행물 정보: 『약학회지』제43권 제4호 (1999년), 423~428쪽, 전체 6쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 1999.08.28

영문 초록

Two polysaccharides, ACP-UMF and ACP-ULF, were purified from the aerial part of Artemisia capillaris by anion-exchange chromatography, ultrafiltration, and gel filtration chromatorgraphy. The polysaccharides appeared to be homogenious from the results of HPLC. The molecular weights of ACP-UMF and ACP-ULF were estimated to be 16305.92 D and 3292.23 D, respectively, by MALDI-TOF MS. The sugar compositions were determined by GC to be arabinose 10.05%, xylose 1.67%, mannose 5.45%, galactose 39.06%, glucose 15.43% for ACP-UMF and arabinose 11.60%, xylose 11.15%, mannose 6.37%, galactose 32.47%, glucose 18.35% for ACP-ULF. A polysaccharide, SP-M, was also purified from Artemisia sylvatica in the same manner as in A. capillaris. The molecular weight of SP-M was determined to be 2462.52 D by MALDI-TOF MS. SP-M consisted mainly of rhamnose 36.49%, arabinose 29.00%, and glucose 19.38%. Incubation of CCl4-intoxicated hepatocytes with ACP-UMF reduced the levels of glutamic pyruvic transaminase(GPT) and cellular malondialdehyde(MDA) to 62.8% and 23.8%. ACP-ULF also reduced the levels of GPT and MDA to 46.1% and 38.1% and SP-M to 38.8% and 26.3%, respectively.

키워드

Artemisia capillaris Artemisia sylvatica polysaccharide liver protective activity.