Silica-based Gel Filtration 크로마토그래피에서의 단백질-실리카 상호작용

• 영문명: Protein-silica Interaction in Silica-based Gel Filtration Chromatography
• 발행기관: 대한약학회
• 저자명: 최중갑(Jung Kap Choi) 유경수(Gyurng Soo Yoo)
• 간행물 정보: 『약학회지』제35권 제6호 (1991년), 461~465쪽, 전체 5쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 1991.12.30

영문 초록

Silica-based gel filtration chromatography has been used to characterize molecular weight of proteins. However, the molecular weight measured by this method was distorted by protein-silica interactions like hydrophobic and electrostatic forces. Therefore, we characterized protein-silica interaction using two forms of phytochrome (124kDa) having different hydrophobicity and surface charge. PH and ionic strength affected the retention time of phytochrome suggesting that electrostatic force is the major interaction between protein and silica surface.

키워드

Silica-based gel filtration chromatography protein-silica interactions.