TGF-β1에 의해 유도되는 세포사멸 신호전달 과정에서 Rac1 GTPase의 역할 규명

• 발행기관: 강원대학교 기초과학연구소
• 저자명: 이신애 김희정 이세신 김병철
• 간행물 정보: 『기초과학연구』제15집, 49~58쪽, 전체 10쪽
• 주제분류: 자연과학 > 자연과학일반
• 파일형태: PDF
• 발행일자: 2004.12.01

영문 초록

  Transforming growth factor (TGF) β1 is a potent inducer of apoptosis in the liver. Recently, c-jun NH₂-terminal kinase (JNK) was shown to be involved in signal transduction in response to TGF-β1. However, the detailed mechanism of TGF-β1-mediated signaling of apoptosis remains largely unknown. In the present study, we investigated the role of Rac1, a member of Rho family GTPases, in the TGF-β1-induced apoptosis of FaO hepatoma cells. Our results showed that TGF-β1-induced apoptosis detected by propidium iodide staining and caspase activation, was significantly blocked in FaO-Rac1N17 cells expressing a dominant negative Rac1 mutant, suggesting a crucial role for Rac1 in TGF-β1 signaling to apoptosis. In addition, we found that TGF-β 1 induced the activation of JNK in a Rac-dependent manner. It thus appears that Rac1 acts upstream of JNK, and that a Rac1-JNK -dependent cascade mediates the TGF-β1-induced apoptosis of FaO cells.

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