2단계 연속배양과 분해세균에 의한 2,4,6-trinitrotoluene (TNT)의 제거

• 발행기관: 강원대학교 기초과학연구소
• 저자명: 김현영 강양미 손윤수 송흥규
• 간행물 정보: 『기초과학연구』제11집, 96~104쪽, 전체 9쪽
• 주제분류: 자연과학 > 자연과학일반
• 파일형태: PDF
• 발행일자: 2000.12.01

영문 초록

Degradation of 2,4,6-trinitrotoluene (TNT) by a white rot fungus lrpex lacteus and activated sludge was investigated in two step bench-scale continuous stirred tank reactors. Inoculum for the first reactor was I. lacteus which already showed a high degradation activity for TNT, and activated sludge from a sewage treatment plant was used as an inoculum for the second reactor. The two-step continuous culture experiment was conducted for 150 days using synthetic feed containing different levels of TNT (10 to 300 mg/L) at room temperature. When a hydraulic retention time of 36 h was maintained, the highest TNT removal rates were 1.66 mg/L/h in first reactor and 0.82 mg/L/h in second reactor during the period of 71~100 days from the start-up. These results indicate the application potential of two-step continuous reactor system for treatment of TNT-containing wastewater. Several TNT-degrading bacteria were isolated from the second reactor, and their biodegradation capabilities were examined. TNT was completely disappeared within 6 hours of further incubation when 100 mg/L of TNT was added into 1-day pregrown C1 bacterial culture. However, this bacterium was unable to use TNT as a sole source of carcon. TNT degration might be accomplished by a cometabolic process using glucose as a growth substrate.

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