학술논문
Protective Effects of Epigallocatechin Gallate after UV Irradiation in Cultured Human Retinal Pigment Epithelial Cells
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- 영문명
- 발행기관
- 대한안과학회
- 저자명
- Seong-Won Yang Byung Rae Lee Jae-Woong Koh
- 간행물 정보
- 『The Korean Journal of Ophthalmology』Vol.21 No.4, 232~237쪽, 전체 6쪽
- 주제분류
- 의약학 > 의학일반
- 파일형태
- 발행일자
- 2007.12.30
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국문 초록
영문 초록
Purpose: To evaluate the protective effects of Epigallocatechin gallate (EGCG) against UV irradiation in cultured human retinal pigment epithelial (RPE) cells.
Methods: UV irradiation was produced by a UV lamp for 30 seconds with an irradiance of 3.3 mW/cm2. After 5 minutes and 1 hour, we administered different concentrations of EGCG (0, 5, 10, 15, 25, 50, 100 uM). The cell count was determined under a microscope using a counting chamber and the cell activity was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay.
Results: The cell count of cultured human RPE cells after UV irradiation was markedly increased in the EGCG administration group, compared with the non-administrated group. The cell activity of the cultured human RPE cells after UV irradiation was markedly increased in the EGCG administration group and was increased in a dose-dependent way as determined by the MTT assay.
Conclusions: The administration of EGCG increased the cell count and the cell activity after UV irradiation in cultured human retinal pigment epithelial cells; this suggests that EGCG provided protection against UV damage in cultured human retinal pigmented epithelial cells.
목차
Materials and Methods
Results
Discussion
키워드
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