Cell Culture Engineering

Gyun Min Lee , Helene Faustrup Kildegaard , Sang Yup Lee , Jens Nielsen , Gregory Stephanopoulos 지음

Wiley-VCH 2019년 09월 10일 출간

작품소개

책소개

Offers a comprehensive overview of cell culture engineering, providing insight into cell engineering, systems biology approaches and processing technology In Cell Culture Engineering: Recombinant Protein Production, editors Gyun Min Lee and Helene Faustrup Kildegaard assemble top class authors to present expert coverage of topics such as: cell line development for therapeutic protein production; development of a transient gene expression upstream platform; and CHO synthetic biology. They provide readers with everything they need to know about enhancing product and bioprocess attributes using genome-scale models of CHO metabolism; omics data and mammalian systems biotechnology; perfusion culture; and much more. This all-new, up-to-date reference covers all of the important aspects of cell culture engineering, including cell engineering, system biology approaches, and processing technology. It describes the challenges in cell line development and cell engineering, e.g. via gene editing tools like CRISPR/Cas9 and with the aim to engineer glycosylation patterns. Furthermore, it gives an overview about synthetic biology approaches applied to cell culture engineering and elaborates the use of CHO cells as common cell line for protein production. In addition, the book discusses the most important aspects of production processes, including cell culture media, batch, fed-batch, and perfusion processes as well as process analytical technology, quality by design, and scale down models. -Covers key elements of cell culture engineering applied to the production of recombinant proteins for therapeutic use -Focuses on mammalian and animal cells to help highlight synthetic and systems biology approaches to cell culture engineering, exemplified by the widely used CHO cell line -Part of the renowned "Advanced Biotechnology" book series Cell Culture Engineering: Recombinant Protein Production will appeal to biotechnologists, bioen

목차

1. A platform technology for therapeutic protein production 2. Cell line development for therapeutic protein production 3. Development of a transient gene expression upstream platform 4. CHO genome: Enhancing product and bioprocess attributes using genome-scale models of CHO metabolism 5. Epigenetics and stability: An investigation on genome heterogeneity and stability and on what controls cell behavior 6. CHO synthetic biology 7. Omics data and mammalian systems biotechnology: an integrative framework for combining in silico modeling and multi-omics datasets in different CHO parental cell lines 8. Mammalian gene editing tools 9. Cell Engineering 10. Metabolite Profiling of Mammalian Cells 11. Application of 13C flux analysis for cell engineering 12. Host cell protein impurity 13. Batch and fed-batch culture 14. Perfusion culture 15. Process analytical technology and quality-by-design for animal cell culture 16. Development of a scale down cell culture model using multivariate analysis as a qualification tool

작가정보

저자(글) Gyun Min Lee

Gyun Min Lee is Professor at the Department of Biological Sciences at KAIST, South Korea, since 2001 and heads the Animal Cell Engineering Laboratory. He studied chemical engineering at Seoul National University, South Korea, and received his Ph.D. from the University of Michigan, USA, in 1990. In 1991, he returned to South Korea and held several positions at KAIST until he became full professor in 2001. From 1998 to 1999, Gyun Min Lee was a visiting scientist at the University of California in San Diego, USA. At present, he is a scientific director at the Novo Nordisk Foundation Center for Biosustainability at the Technical University of Denmark. He received several national awards and published more than 200 publications in the field of cell culture and protein production. Helene Faustrup Kildegaard is a senior researcher and Co-PI for the CHO Cell Line Engineering and Design section at the Novo Nordisk Foundation Center for Biosustainability at the Technical University of Denmark (DTU). In 2009, she received her Ph.D. in molecular and cell biology from DTU, where she held several positions afterwards. From 2003 to 2004, she studied abroad at Michigan Technological University, USA, and was a visiting researcher at the University of Natural Resources and Life Sciences Vienna, Austria, in 2016.

저자(글) Helene Faustrup Kildegaard

저자(글) Sang Yup Lee

저자(글) Jens Nielsen

저자(글) Gregory Stephanopoulos