MALDI MS

Franz Hillenkamp 지음

Wiley-Blackwell 2013년 10월 08일 출간

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This authoritative book on MALDI MS, now finally available in its second edition and edited by one of its inventors, gives an in-depth description of the many different applications, along with a detailed discussion of the technology itself.Thoroughly updated and expanded, with contributions from key players in the field, this unique book provides a comprehensive overview of MALDI MS along with itspossibilities and limitations.The initial chapters deal with the technology and the instrumental setup, followed by chapters on the use of MALDI MS in protein research (including proteomics), genomics, glycomics and lipidomics. The option of MALDI-MS for the analysis of polymers and small molecules are also covered in separate chapters, while new to this edition is a section devoted to the interplay of MALDI MS and bioinformatics.A much-needed practical and educational asset for individuals, academic institutions and companies in the field of bioanalytics.

목차

Preface to the Second Edition XIList of Contributors XIII1 The MALDI Process and Method 1Franz Hillenkamp, Thorsten W. Jaskolla, and Michael Karas1.1 Introduction 11.2 Analyte Incorporation 41.3 Absorption of the Laser Radiation 61.4 The Ablation/Desorption Process 91.5 Ionization 131.6 Fragmentation of MALDI Ions 171.7 MALDI of Noncovalent Complexes 211.8 The Optimal Choice of Matrix: Sample Preparation 241.8.1 Surface Preparation 271.8.2 Anchor Sample Plates 271.8.3 Matrix Additives and Influence of the Sample Plate Surface 29Abbreviations 30References 312 MALDI Mass Spectrometry Instrumentation 41Peter B. O&'Connor, Klaus Dreisewerd, Kerstin Strupat, and Franz Hillenkamp2.1 Introduction 412.2 Lasers for MALDI-MS 422.3 Fragmentation of MALDI Ions 482.3.1 MALDI at Elevated Pressure 482.3.2 Tandem Mass Spectrometry of MALDI Ions 492.4 Mass Analyzers 522.4.1 Axial TOF Mass Spectrometers 532.4.2 Reflectron TOF Mass Spectrometers 552.4.3 Tandem TOF Mass Spectrometers 562.4.4 Orthogonal TOF Mass Analyzers 602.4.5 Tandem Mass Spectrometry in oTOF Mass Analyzers 612.4.6 Ion Detectors and Data Processing in MALDI-TOF Analyzers 622.5 Fourier Transform Ion Cyclotron Resonance Mass Spectrometers 642.5.1 Tandem Mass Spectrometry on FTICR Mass Spectrometers 702.6 Quadrupole Ion Trap Mass Spectrometers 722.6.1 RF-Only Ion Guides and LIT Mass Spectrometers 772.6.2 Tandem Mass Spectrometry on QIT Mass Spectrometers 772.7 Hybrid Mass Spectrometers 792.7.1 Quadrupole TOF Mass Spectrometers 792.7.2 Quadrupole FT Mass Spectrometers 802.7.3 QIT-TOF Mass Spectrometers 822.7.4 Ion Mobility oTOF Mass Spectrometers 832.7.5 Orbitrap 872.8 Future Directions 92Def

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저자(글) Franz Hillenkamp

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Franz Hillenkamp is Professor Emeritus at the University of Muuml;nster, Germany. He holds a MS degree in electrical engineering from Purdue University, USA, and a PhD from the Technical University of Munich, Germany. Before he was appointed Professor of Biophysics and Medical Physics at Muuml;nster in 1986, he held a professorship at the University of Frankfurt, Germany. During the 1980s,he developed the now world famous MALDI technique that was later on shown to be highly useful for the analysis of biomolecules. For his ground-breaking work on mass spectrometry methods, Professor Hillenkamp has received numerous awards, among them the Thomson Medal of the Mass Spectrometry Society, the Fresenius Medal of the German Chemical Society, and the Bergman Medal of theSwedish Chemical Society.Jasna Peter-Katalinic is Professor at the University of Rijeka, Croatia, and former Associate Professor of Biophysics at the University of Muuml;nster, Germany. She wasborn and educated in Zagreb, Croatia, and obtained her PhD in chemistry at the University of Zuuml;rich, Switzerland. After the postdoc time at the Texas A+M University, USA, she obtained the habilitation in physiological chemistry from the University of Bonn, Germany. She pioneered the introduction of modern massspectrometric methods to structural glycobiology/ glycomics, as described in more than 250 publications. Her current interests are in the Human Glycoproteome Initiative and Nanobioanalytics. She was the first recipient of the Life Science Award from the German Society of Mass Spectrometry in 2002.