Handbook of RNA Biochemistry

Roland K. Hartmann , Albrecht Bindereif , Astrid Schön , Eric Westhof 지음

Wiley-VCH 2015년 03월 31일 출간

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Preface XXXV List of Contributors XXXVII Part I RNA Synthesis and Detection 1 1 Enzymatic RNA Synthesis Using Bacteriophage T7 RNA Polymerase 3Markus Gößringer, Dominik Helmecke, Karen Köhler, Astrid Schön, Leif A. Kirsebom, Albrecht Bindereif, and Roland K. Hartmann 2 Production of RNAs with Homogeneous 5'- and 3'-Ends 29Mario Mörl and Roland K. Hartmann 3 RNA Ligation 45Janne J. Turunen, Liudmila V. Pavlova, Martin Hengesbach, Mark Helm, Sabine Müller, Roland K. Hartmann, and Mikko J. Frilander 4 Northern Blot Detection of Small RNAs 89Benedikt M. Beckmann, Arnold Grünweller, and Roland K. Hartmann 5 Rapid, Non-Denaturing, Large-Scale Purification of In Vitro Transcribed RNA Using Weak Anion-Exchange Chromatography 105Laura E. Easton, Yoko Shibata, and Peter J. Lukavsky 6 3'-Terminal Attachment of Fluorescent Dyes and Biotin 117Dagmar K. Willkomm and Roland K. Hartmann 7 Chemical RNA Synthesis, Purification, and Analysis 129Brian S. Sproat 8 Modified RNAs as Tools in RNA Biochemistry 151Thomas E. Edwards and Snorri Th. Sigurdsson Part II Structure Determination 173 9 Direct Determination of RNA Sequence and Modification by Radiolabeling Methods 175Olaf Gimple and Astrid Schön 10 Probing RNA Structure In Vitro with Enzymes and Chemicals 205Anne-Catherine Helfer, C&e'dric Romilly, Cl&e'ment Chevalier, Efthimia Lioliou, Stefano Marzi, and Pascale Romby 11 Probing RNA Solution Structure by Photocrosslinking: Incorporation of Photoreactive Groups at RNA Termini and Determination of Crosslinked Sites by Primer Extension 231Michael E. Harris 12 Terbium(III) Footprinting as a Probe of RNA Structure and Metal Binding Sites 255Dinari A. Harris, Gabrielle

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저자(글) Roland K. Hartmann

Roland K. Hartmann is Professor of Pharmaceutical Chemistry at the Philipps-Universität Marburg (Germany). He studied Biochemistry at the Freie Universität Berlin where in 1988 he received the Ernst Reuter award for outstanding Ph.D. theses. His research interests include natural ribozymes, particularly ribonuclease P, interactions of RNAs with proteins and small ligands, aptamers, antisense and RNA interference.Albrecht Bindereif is Professor of Biochemistry at the University of Giessen (Germany) since 1999. He studied Biochemistry in Tübingen and at the University of California, Berkeley. His postdoctoral work was done in Michael Green's laboratory at Harvard University. His research interests focus on the mechanism and regulation of mRNA splicing, both in the mammalian system and in trypanosomes.Astrid Schön received her PhD from the Institute of Biochemistry at the University of Würzburg, and was a graduate fellow and postdoctoral associate with Dieter Söll at Yale University. She is currently a lecturer at the Institute of Biochemistry, University of Leipzig (Germany). Her research is focused on RNA metabolism and RNA-protein interactions, and the evolution of complex RNA enzymes.Eric Westhof is Professor of Biophysics at the Universit&e' Louis Pasteur in Strasbourg (France) since 1988. He studied in Liege and Regensburg and was a Fulbright-Hays Research Fellow at the University of Wisconsin with M. Sundaralingam. His research is centered on the relationships between sequence, structure, evolution and functions of RNA molecules.

저자(글) Albrecht Bindereif

저자(글) Astrid Schön

저자(글) Eric Westhof