High-Throughput Screening Methods in Toxicity Testing

Pablo Steinberg 지음

Wiley 2013년 02월 26일 출간

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Explores the benefits and limitations of the latest high#45;throughput screening methodsWith its expert coverage of high#45;throughput in vitro screening methods for toxicity testing, this book makes it possible for researchers to accelerate and streamline the evaluation and risk assessment of chemicals and drugs for toxicity. Moreover, it enables them to comply with the latest standards set forth by the U.S. National Research Council#39;s #34;Toxicity Testing in the 21st Century: A Vision and Strategy#34; and the E.U.#39;s REACH legislation. Readers will discover a variety of state#45;of#45;the#45;science, high#45;throughput screening methods presented by a group of leading authorities in toxicology and toxicity testing.High#45;Throughput Screening Methods in Toxicity Testing is divided into five parts:General aspects, including predicting the toxicity potential of chemicals and drugs via high#45;throughput bioactivity profilingAssessing different cytotoxicity endpointsAssessing DNA damage and carcinogenesisAssessing reproductive toxicity, cardiotoxicity, and haematotoxicityAssessing drug metabolism and receptor#45;related toxicityEach chapter describes method principles and includes detailed information about data generation, data analysis, and applications in risk assessment. The authors not only enumerate the advantages of each high#45;throughput method over comparable conventional methods, but also point out the high#45;throughput method#39;s limitations and potential pitfalls. In addition, the authors describe current research efforts to make high#45;throughput toxicity screening even more cost effective and streamlined.Throughout the book, readers will find plenty of figures and illustrations to help them understand and perform the latest high#45;throughput toxicity screening met

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PREFACE ixCONTRIBUTORS xiPART I GENERAL ASPECTS1 ToxCast: Predicting Toxicity Potential Through High-Throughput Bioactivity Profiling 3Keith A. Houck, Ann M. Richard, Richard S. Judson, Matthew T. Martin, David M. Reif, and Imran Shah2 High-Throughput Toxicity Testing in Drug Development: Aims, Strategies, and Novel Trends 33Willem G.E.J. Schoonen, Walter M.A. Westerink, Femke M. van de Water, and Horbach G. Jean3 Incorporating Human Dosimetry and Exposure Information with High-Throughput Screening Data in Chemical Toxicity Assessment 77Barbara A. Wetmore and Russell S. Thomas4 The Use of Human Embryonic Stem Cells in High-Throughput Toxicity Assays 97Xin Huang, Dan-yan Zhu, and Yi-jia LouPART II HIGH-THROUGHPUT ASSAYS TO ASSESS DIFFERENT CYTOTOXICITY ENDPOINTS5 High-Throughput Screening Assays for the Assessment of Cytotoxicity 109Andrew L. Niles, Richard A. Moravec, Tracy J. Worzella, Nathan J. Evans, and Terry L. Riss6 High-Throughput Flow Cytometry Analysis of Apoptosis 129Francesca de Giorgi and Franc¸ois Ichas7 High Content Imaging-Based Screening for Cellular Toxicity Pathways 143Bram Herpers and Bob van de Water8 The Keratinosens Assay: A High-Throughput Screening Assay to Assess Chemical Skin Sensitization 159Andreas Natsch9 High-Throughput Screening Assays to Assess Chemical Phototoxicity 177Satomi Onoue, Yoshiki Seto, and Shizuo YamadaPART III HIGH-THROUGHPUT ASSAYS TO ASSESS DNA DAMAGE AND CARCINOGENESIS10 Ames IITM and Ames Liquid Format Mutagenicity Screening Assays 193Kamala Pant11 High-Throughput Bacterial Mutagenicity Testing: VitotoxTM Assay 213Luc Verschaeve12 Genotoxicity and Carcinogenicity: Regulatory and Novel Test Methods 233Walter M.A. Westerink, Joe C.R.

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저자(글) Pablo Steinberg

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PABLO STEINBERG, PhD, is Full Professor for Food Toxicology and Replacement/Complementary Methods to Animal Testing as well as Director of the Institute for Food Toxicology and Analytical Chemistry at the University of Veterinary Medicine Hannover. Prior to that, Dr. Steinberg was director of the Institute of Nutritional Science at the University of Potsdam. He has also held research positions at the University of Mainz.