Protein and Peptide Mass Spectrometry in Drug Discovery

Michael L. Gross 지음

Wiley 2011년 09월 26일 출간

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The book that highlights mass spectrometry and its application in characterizing proteins and peptides in drug discoveryAn instrumental analytical method for quantifying the mass and characterization of various samples from small molecules to large proteins, mass spectrometry (MS) has become one of the most widely used techniques for studying proteins and peptides over the last decade. Bringing together the work of experts in academia and industry, Protein and Peptide Mass Spectrometry in Drug Discovery highlights current analytical approaches, industry practices, and modern strategies for the characterization of both peptides and proteins in drug discovery.Illustrating the critical role MS technology plays in characterizing target proteins and protein products, the methods used, ion mobility, and the use of microwave radiation to speed proteolysis, the book also covers important emerging applications for neuroproteomics and antigenic peptides. Placing an emphasis on the pharmaceutical industry, the book stresses practice and applications, presenting real-world examples covering the most recent advances in mass spectrometry, and providing an invaluable resource for pharmaceutical scientists in industry and academia, analytical and bioanalytical chemists, and researchers in protein science and proteomics.

목차

PREFACE xvCONTRIBUTORS xviiPART I METHODOLOGY 11 Ionization Methods in Protein Mass Spectrometry 3Ismael Cotte-Rodriguez, Yun Zhang, Zhixin Miao, and Hao Chen1.1 History of the Development of Protein Mass Spectrometry 41.2 Laser-Based Ionization Methods for Proteins 51.3 Spray-Based Ionization Methods for Proteins 131.4 Ambient Ionization Methods 201.5 Conclusions 30Acknowledgments 30References 302 Ion Activation and Mass Analysis in Protein Mass Spectrometry 43Cheng Lin and Peter O&-Connor2.1 Introduction 432.2 Ion Activation and Tandem MS Analysis 462.3 Mass Analyzers 59References 813 Target Proteins: Bottom-up and Top-down Proteomics 89Michael Boyne and Ron Bose3.1 Mass Spectral Approaches to Targeted Protein Identification 893.2 Bottom-up Proteomics 903.3 Top-down Approaches 963.4 Next-Generation Approaches 98References 994 Quantitative Proteomics by Mass Spectrometry 101Jacob Galan, Anton Iliuk, and W. Andy Tao4.1 Introduction 1014.2 In-Cell Labeling 1054.3 Quantitation via Isotopic Labeling of Proteins 1074.4 Quantitation via Isotopic Labeling on Peptides 1124.5 Label-Free Quantitation 1164.6 Conclusions 119Acknowledgment 120References 1205 Comparative Proteomics by Direct Tissue Analysis Using Imaging Mass Spectrometry 129Michelle L. Reyzer and Richard M. Caprioli5.1 Introduction 1295.2 Conventional Comparative Proteomics 1305.3 Comparative Proteomics Using Imaging MS 1315.4 Conclusions 136Acknowledgments 137References 1376 Peptide and Protein Analysis Using Ion Mobility&~Mass Spectrometry 139Jeffrey R. Enders, Michal Kliman, Sevugarajan Sundarapandian, and John A. McLean6.1 Ion Mobility&~Mass Spectrometry: Instrumentation and Separation Selectivity 1396.2 Characterizing and Interpreting Peptide and Protein Struct

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"This book will be a valuable reference as it contains plenty of depth and substance to be of interest to experienced practitioners of mass spectrometry and related techniques, but is still accessible to pharmaceutical researchers who want to learn more about MS technologies and its applications."& (American Society for Mass Spectrometry, 1 July 2012)&&

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저자(글) Michael L. Gross

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Michael L. Gross, PhD, is a Professor in the Departments of Chemistry, Medicine, and Immunology at Washington University in St. Louis. He is also Principal Investigator at the National Institutes of Health (NIH) Research Resource in Mass Spectrometry.Guodong Chen, PhD, is Principal Scientist in Bioanalytical and Discovery Analytical Sciences at Bristol-Myers Squibb in Princeton, New Jersey. He heads a mass spectrometry group in support of drug discovery as well as development programs in small molecule pharmaceuticals and biologics.Birendra N. Pramanik, PhD, was a Distinguished Fellow at Schering-Plough Research Institute (SPRI), where he directed the spectroscopy, mass spectrometry, and NMR programs.