Fluorescent Proteins

Kevin F. Sullivan 지음

elsevier 2007년 12월 14일 출간

작품소개

책소개

This new edition of Fluorescent Proteins presents current applications of autofluorescent proteins in cell and molecular biology authored by researchers from many of the key laboratories in the field. Starting from a current review of the broad palette of fluorescent proteins available, several chapters focus on key autofluorescent protein variants, including spectral variants, photodynamic variants as well as chimeric FP approaches. Molecular applications are addressed in chapters that detail work with single molecules, approaches to generating protein fusions and biosensors as well as analysis of protein-protein interactions in vivo by FRET, fluorescence polarization and fluorescence cross correlation techniques. A number of approaches to in vivo dynamics are presented, including FRAP, photoactivation, and 4-dimensional microscopy. Behavior of spindle components, membrane proteins, mRNA trafficking as well as analysis of cell types in tissues and in development are detailed and provide models for a wide variety of experimental approaches. In addition, several chapters deal directly with the computational issues involved in processing multidimensional image data and using fluorescent imaging to probe cellular behavior with quantitative modeling. This volume brings together the latest perspective and techniques on fluorescent proteins and will be an invaluable reference in a wide range of laboratories.

목차

1. Fluorescent ProteinsIan M. Dobie, Noel F. Lowndes and Kevin F. Sullivan2. Functional Fusion Proteins by Random Transposon-Based GFP InsertionRobert Mealer, Heather Butler & Thomas Hughes3. Fluorescent Proteins for Photoactivation ExperimentsJennifer Lippincott-Schwartz and George H. Patterson4. Design and Optimization of Genetically Encoded Fluorescent Biosensors: Gtpase BiosensorsLouis Hodgson and Klaus M. Hahn5. Fast 4D MicroscopyJ. R. De Mey, P. Kessler, J. Dompierre, F. P. Cordeli챔res, A. Dieterlen, J. -L Vonesch, and J. ？？B. Sibarita6. Single molecule imaging of fluorescent proteinsAdam D. Douglass and Ronald D. Vale7. Counting Kinetochore Protein Numbers in Budding Yeast Using Genetically Encoded Fluorescent ProteinsAjit Joglekar, E. D. Salmon, Kerry Bloom8. Fluorescent Protein Applications in PlantsR. Howard Berg, Roger N. Beachy9. Expression and Imaging of Fluorescent Proteins in the C. elegans Gonad and Early EmbryoRebecca A. Green, Anjon Audhya, Andrei Pozniakovsky, Alexander Dammermann, Hayley Pemble, Joost Monen, Nathan Portier, Anthony Hyman, Arshad Desai and Karen Oegema10. Fluorescent Proteins in Zebrafish Cell and Developmental BiologyH. William Detrich, III11. Identifying and Quantitating Neural Stem and Progenitor Cells in the Adult BrainJuan Manuel Encinas and Grigori Enikolopov 12. Using Fluorescent Proteins to Study mRNA Trafficking in Living CellsEmmanuelle Querido and Pascal Chartrand13. Visualising mRNA Localisation and Local Protein Translation in NeuronsRalf Dahm, Manuel Zeitelhofer, Bernhard G철tze, Michael A. Kiebler and Paolo Macchi14. Quantitative FRAP in Analysis of Molecular Binding Dynamics In Vivo James G. McNally 15. Quantitative and Qualitative Analysis of Plant Membrane Traffic UsingFluorescent ProteinsMarketa Samalova, Mark Fricker, and Ian Moore.

작가정보

저자(글) Kevin F. Sullivan