Cell Biology, Four-Volume Set

Julio E. Celis , Nigel Carter , Kai Simons , J. Victor Small , Tony Hunter 지음

elsevier 2005년 11월 16일 출간

작품소개

책소개

This four-volume laboratory manual contains comprehensive state-of-the-art protocols essential for research in the life sciences. Techniques are presented in a friendly step-by-step fashion, providing useful tips and potential pitfalls. The important steps and results are beautifully illustrated for further ease of use. This collection enables researchers at all stages of their careers to embark on basic biological problems using a variety of technologies and model systems.

This thoroughly updated third edition contains 165 new articles in classical as well as rapidly emerging technologies.

Topics covered include:
* Cell and Tissue Culture: Associated Techniques, Viruses, Antibodies, Immunocytochemistry (Volume 1)
* Organelle and Cellular Structures, Assays (Volume 2)
* Imaging Techniques, Electron Microscopy, Scanning Probe and Scanning Electron Microscopy, Microdissection, Tissue Arrays, Cytogenetics and In Situ Hybridization, Genomics and Transgenic Knockouts and Knock-down Methods (Volume 3)
* Transfer of Macromolecules, Expression Systems, Gene Expression Profiling (Volume 4)

* Indispensable bench companion for every life science laboratory
* Provides the latest information on the plethora of technologies needed to tackle complex biological problems
* Includes numerous illustrations, some in full color, supporting steps and results

목차

Contents of Volume 1:
Part A. Cell and Tissue Culture: Associated Techniques.
Section 1. General Techniques.
Section 2. Culture of Specific Cell Types: Stem Cells.
Section 3. Culture of Specific Cell Types: Haemopoietic, Mesenchymal, and Epithelial.
Section 4. Differentiation and Reprogramming of Somatic Cells.
Section 5. Immortalisation.
Section 6. Somatic Cell Hybrids.
Section 7. Cell Separation Techniques.
Section 8. Cell Cycle Analysis.
Section 9. Cytotoxic and Cell Growth Assays.
Section 10. Apoptosis.
Section 11. Assays of Cell Transformation, Tumorigenesis, Invasion and Wound Healing.
Section 12. Electrophysiological Methods.
Section 13. Organ Cultures.

Part B. Viruses.
Section 14. Growth and Purification of Viruses.

Part C. Antibodies.
Section 15. Production and Purification of Antibodies.

Part D. Immunocytochemistry.
Section 16. Immunofluoresence.

Part E. Appendix.

Contents of Volume 2:
Part A. Organelles and Cellular Structures.
Section 1. Isolation: Plasma Membrane, Organelles, and Cellular Structures.
Section 2. Vital Staining of Cells/Organelles.
Section 3. Protein Purification.

Part B. Assays.
Section 4. Endocytic and Exocytic Pathways.
Section 5. Membranes.
Section 6. Mitochondria.
Section 7. Nuclear Transport.
Section 8. Chromatin Assembly.
Section 9. Signal Transduction Assays.
Section 10. Assays and Models of in Vitro and in Vitro Motility.
Section 11. Mechanical Stress in Single Cells.

Part C. Appendix.

Contents of Volume 3.
Part A. Imaging Techniques.
Section 1. Light Microscopy.
Section 2. Digital Video Microscopy.
Section 3. Confocal Microscopy of Living Cells and Fixed Cells.
Section 4. Fluorescent Microscopy of Living Cells.
Section 5. Use of Fluorescent Dyes for Studies of Intracellular Physiological Parameters.
Section 6. Digital Image Processing, Analysis, Storage,

출판사 서평

"4 Stars - These 4 volumes provide a modern, near encyclopedic review of cell biology protocols."
--Gregory Jay Bix, Thomas Jefferson University in DOODY'S (May 2006)

Praise for the Previous Edition:
"The terms bible and encyclopedic collection are often used of scientific works aspiring to present to the scientific community all the branches of a discipline. Often these terms are misleading! Here they are paradigmatic... could not be more accurate and user-friendly... The book is not only helpful for writing papers, but invaluable for bench work... a masterpiece among scientific publications."
--EUROPEAN JOURNAL OF HISTOCHEMISTRY
"This compendium is a welcome addition to the cell biology laboratory, assembling a wide array of techniques of value to cell biologists...Contributions are by recognized experts in their fields, in some cases the originator of the technique or procedure.
--CHOICE

작가정보

저자(글) Julio E. Celis

저자(글) Nigel Carter

저자(글) Kai Simons

저자(글) J. Victor Small

저자(글) Tony Hunter